(HeLa treated by Starvatio and IGF, rat brain cells, Pig lung, 293T, DF-1) Anti-actin antibody (1:40000, Milipore) is used as a loading control. TE-1, SW480, NIH/3T3, 293T, Mouse brain, Mouse testis, mose lung, rat brain 3.7 Validation: Western blotting Candidate circadian biomarkers identified by. The diluted antibody can be stored at -20℃ without aliquot. Put 5ul AC026 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition Human, Mouse, Rat, Chicken, Zebrafish, Pig Protein Kinase C Signaling Pathway Pathway.Specificity for gamma actin determined by 2D gel electrophoresis. Blocking with purified milk proteins preferred over commerical concentrated milk solutions. Cell Intrinsic Innate Immunity Signaling Pathway Western Blot: 1:500-1:2,000 or greater non-specific bands can occur when primary antibody is overloaded.Secondary antibody: HRP Goat Anti-Rabbit IgG. Jak-Stat-IL-6 Receptor Signaling Pathway Western blot analysis of extracts of various cell lines, using -actin antibody (AC026) at 1:100000 dilution.Epigenetic writers and erasers of core Histones.

Anti actin antibody western blot